Journal: Acta neuropathologica communications

Article Title: BDNF augmentation reverses cranial radiation therapy-induced cognitive decline and neurodegenerative consequences.

doi: 10.1186/s40478-024-01906-9

Figure Lengend Snippet: Fig. 2 Riluzole treatment restores hippocampal BDNF in the irradiated hippocampus. 10–12 weeks old WT male mice received cranial radiation therapy (RT) followed by riluzole (RZ) treatment (13 mg/kg) in drinking water for 6–7 weeks. An ELISA-based quantification of BDNF from the micro-dissected hippocampus showed RT-induced reductions in the RT + Vehicle group. Importantly, RZ treatment in the cranially irradiated mice showed significant restoration of BDNF levels. Data are presented as mean ± SEM (N = 6–10 mice per group). P values were derived from two-way ANOVA and Bonferroni’s multiple comparisons test

Article Snippet: BDNF levels were quantified using a commercially available ELISA kit (E-EL-M0203, Elabscience Biotechnology) and uncoated ELISA plates (Nunc MaxiSorp, Biolegend).

Techniques: Irradiation, Enzyme-linked Immunosorbent Assay, Derivative Assay

Journal: Journal of Neuroimmunology

Article Title: PKR mediated regulation of inflammation and IL-10 during viral encephalomyelitis

doi: 10.1016/j.jneuroim.2014.02.012

Figure Lengend Snippet: PKR deficiency results in increased perivascular accumulation of CD4 T cells. (A) Localization of CD4 T cells was assessed at days 7 and 10 p.i. by dual staining of brain sections for laminin (green) and CD4 (red) and analyzed by confocal microscopy. Bar graphs show average of CD4 T cells in perivascular space and in brain parenchyma of 6–9 fields/brain with n = 6 mice/group/time-point ± SEM. (B) Staining of brain sections at day 7 p.i. for PKR-p (green), CD4 (red) and nuclei (DAPI, blue) was analyzed by florescent microscopy. Images for both panels are representative of 6–9 fields/brain with n = 6 mice/group/time-point. *p ≥ .05.

Article Snippet: The distribution of CD4 T cells in brain parenchyma and perivascular space was determined by staining brain sections with rabbit anti-mouse laminin Ab (Cedarlane Laboratories, Ontario, Canada) and rat anti-mouse CD4 mAb (BD Pharmingen).

Techniques: Staining, Confocal Microscopy, Microscopy